	US 12,077,769 B2
	ZmWAK-RLK protein related to gray leaf spot resistance, and encoding gene and application thereof
Mingliang Xu, Beijing (CN); Tao Zhong, Beijing (CN); Xingming Fan, Beijing (CN); Mang Zhu, Beijing (CN); Yan Zhang, Beijing (CN); Ling Xu, Beijing (CN); and Li Liu, Beijing (CN)
Assigned to CHINA AGRICULTURAL UNIVERSITY, Beijing (CN)
Appl. No. 17/433,159
Filed by CHINA AGRICULTURAL UNIVERSITY, Beijing (CN)
PCT Filed Feb. 24, 2020, PCT No. PCT/CN2020/076320 § 371(c)(1), (2) Date Aug. 23, 2021, PCT Pub. No. WO2020/173403, PCT Pub. Date Sep. 3, 2020.
Claims priority of application No. 201910140479.1 (CN), filed on Feb. 26, 2019.
Prior Publication US 2022/0177907 A1, Jun. 9, 2022
Int. Cl. C12N 15/82 (2006.01)

CPC C12N 15/8282 (2013.01)

15 Claims

1. A nucleic acid molecule, which encodes a protein comprising:

(i) the amino acid of SEQ ID NO: 1;

(ii) the amino acid of SEQ ID NO: 7;

(iii) the amino acid of SEQ ID NO: 1 or SEQ ID NO: 7 further comprising a peptide tag linked to an N-terminus or/and a C-terminus of SEQ ID NO: 1 or SEQ ID NO: 7; or

(iv) an amino acid sequence that differs from SEQ ID NO: 1 or SEQ ID NO: 7 by a conservative substitution of one of the amino acid residues of SEQ ID NO: 1 or SEQ ID NO: 7;

wherein the nucleic acid molecule is operably linked to a heterologous promoter or a heterologous regulatory element.