US 12,077,768 B2
Plants having increased tolerance to herbicides
Raphael Aponte, Mannheim (DE); Stefan Tresch, Ludwigshafen (DE); Matthias Witschel, Ludwigshafen (DE); Jens Lerchl, Limburgerhof (DE); Dario Massa, Limburgerhof (DE); Tobias Seiser, Ludwigshafen (DE); Thomas Mietzner, Annweiler (DE); Jill Marie Paulik, Durham, NC (US); and Chad Brommer, Raleigh, NC (US)
Assigned to BASF AGRO B.V., Arnhem (NL)
Filed by BASF AGRO B.V., Arnhem (NL)
Filed on Apr. 15, 2022, as Appl. No. 17/721,822.
Application 17/721,822 is a continuation of application No. 16/392,207, filed on Apr. 23, 2019, granted, now 11,306,322.
Application 16/392,207 is a continuation of application No. 15/105,270, granted, now 10,308,953, previously published as PCT/IB2014/067018, filed on Dec. 17, 2014.
Claims priority of provisional application 61/917,360, filed on Dec. 18, 2013.
Prior Publication US 2022/0315944 A1, Oct. 6, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/82 (2006.01); A01N 37/10 (2006.01); A01N 43/54 (2006.01); A01N 43/653 (2006.01); A01N 43/84 (2006.01); C12N 9/02 (2006.01); C12N 9/10 (2006.01)
CPC C12N 15/8274 (2013.01) [A01N 37/10 (2013.01); A01N 43/54 (2013.01); A01N 43/653 (2013.01); A01N 43/84 (2013.01); C12N 9/001 (2013.01); C12N 9/1059 (2013.01); C12Y 103/03004 (2013.01)] 10 Claims
 
1. A method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of:
a) providing, at said site, a plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding a mutated protoporphyrinogen oxidase (mutated-PPO) which is resistant or tolerant to a “PPO inhibiting herbicide”, and which comprises (1) the amino acid sequence of SEQ ID NO: 30 or 31 with a double amino acid substitution selected from the group consisting of R130A/F433V; L410Q/F433V; L410E/F433M; and L410Q/F433M or (2) the amino acid sequence of SEQ ID NO: 117 with a double amino acid substitution selected from the group consisting of R99A/F394V; L371E/F394V; L371Q/F394V; L371E/F394M; and L371Q/F394M; and
b) applying to said site an effective amount of said herbicide.