	US 12,077,767 B2
	Altering thermoresponsive growth in plants via genome editing of phytochrome interacting factor 4 (PIF4) regulatory elements
Chloé Zubieta, Grenoble (FR); and Aditya Nayak, Grenoble (FR)
Assigned to COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES, Paris (FR); and CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS), Paris (FR)
Filed by COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES, Paris (FR); and CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS), Paris (FR)
Filed on Mar. 17, 2020, as Appl. No. 16/820,838.
Claims priority of application No. 19305324 (EP), filed on Mar. 18, 2019.
Prior Publication US 2020/0299713 A1, Sep. 24, 2020
Int. Cl. C12N 15/82 (2006.01)

CPC C12N 15/8271 (2013.01) [C12N 15/8213 (2013.01); C12N 15/8218 (2013.01)]

17 Claims

1. A genetically engineered plant wherein a G-box motif of the Phytochrome Interacting Factor 4 (PIF4) promoter is inactivated, wherein the G-box motif has a core sequence consisting of the nucleic acid sequence of SEQ ID NO: 1 before inactivation and is within 1 kb of the LUX binding site of the PIF4 promoter and within 2 kilobases (kb) of the PIF4 gene start codon, wherein the LUX binding site has a core sequence consisting of the nucleic acid sequence of SEQ ID NO: 2; wherein the inactivation of the G-box motif leads to the decrease or inhibition of the binding of the PIF4 protein on the G-box motif of the PIF4 promoter, wherein the plant has a thermosensory response which is inhibited so that the plant is tolerant to an increase in ambient temperature, and wherein the genetically engineered plant is from the family Brassicaceae.