	US 12,077,764 B2
	Delivery of developmental regulators to plants for the induction of meristematic tissue with genetic alterations
Daniel F. Voytas, Falcon Heights, MN (US); Ryan A. Nasti, Berkeley, CA (US); and Michael F. Maher, St. Paul, MN (US)
Assigned to Regents of the University of Minnesota, Minneapolis, MN (US)
Filed by Regents of the University of Minnesota, Minneapolis, MN (US)
Filed on Mar. 3, 2023, as Appl. No. 18/178,290.
Application 18/178,290 is a continuation of application No. 17/255,305, granted, now 11,608,506, previously published as PCT/US2019/039297, filed on Jun. 26, 2019.
Claims priority of provisional application 62/690,165, filed on Jun. 26, 2018.
Prior Publication US 2023/0265447 A1, Aug. 24, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/82 (2006.01); A01H 4/00 (2006.01); C12Q 1/6895 (2018.01)

CPC C12N 15/8229 (2013.01) [A01H 4/008 (2013.01); C12N 15/8213 (2013.01); C12Q 1/6895 (2013.01); C12Q 2600/13 (2013.01)]

19 Claims

1. A method for generating plant tissue comprising one or more genetic modifications of interest, the method comprising:

(a) introducing into plant cells (i) a nucleic acid construct encoding one or more developmental regulators that, when expressed in the plant cells, induce meristem formation from the plant cells, and (ii) a nucleic acid construct comprising one or more sequences that, when expressed, edit the plant cell DNA to introduce one or more genetic modifications of interest, wherein the introducing comprises viral infection; and

(b) deriving de novo tissue from plant cells identified as having the one or more genetic modifications of interest.