US 12,077,763 B2
RNA-based control of powdery mildew
Adriana Puralewski, Cary, NC (US); Christopher Lawrence, Blacksburg, VA (US); Upendra Kumar Devisetty, Morrisville, NC (US); Sambit Kumar Mishra, Cary, NC (US); Krishnakumar Sridharan, Cary, NC (US); Lance E. Cadle Davidson, Geneva, NY (US); Anna N. Underhill, Geneva, NY (US); Yufeng Fang, Durham, NC (US); and Wimalanathan Kokulapalan, Cambridge, MA (US)
Assigned to GREENLIGHT BIOSCIENCES, INC., Lexington, MA (US); and UNITED STATES, AS REPRESENTED BY THE SECRETARY OF AGRICULTURE, Beltsville, MD (US)
Filed by Greenlight Biosciences, Inc., Lexington, MA (US); and United States of America, as represented by the Secretary of Agriculture, Beltsville, MD (US)
Filed on Mar. 8, 2022, as Appl. No. 17/689,598.
Claims priority of provisional application 63/158,293, filed on Mar. 8, 2021.
Prior Publication US 2022/0290170 A1, Sep. 15, 2022
Int. Cl. C12N 15/82 (2006.01); C12N 15/113 (2010.01)
CPC C12N 15/8218 (2013.01) [C12N 15/113 (2013.01); C12N 15/8286 (2013.01)] 12 Claims
 
1. A composition for controlling E. necator, comprising a double-stranded RNA molecule that causes mortality, suppression of growth, decrease in virulence or pathogenicity or decrease in propagation/reproductive capacity in E. necator when transfected or contacted to said E. necator, said double-stranded RNA molecule having a first strand and a second strand, wherein the first strand and second strand are each about 600 nucleotides or less in length and the first strand comprises a nucleotide sequence comprising SEQ ID NO: 215 and the second strand comprises a nucleotide sequence that is complementary to SEQ ID NO: 215.