US 12,077,750 B2
Nanocaged enzymes with enhanced catalytic activity and increased stability
Jinglin Fu, Tempe, AZ (US); Zhao Zhao, Boston, MA (US); Neal Woodbury, Tempe, AZ (US); and Hao Yan, Chandler, AZ (US)
Assigned to Arizona Board of Regents on behalf of Arizona State University, Scottsdale, AZ (US)
Filed by Arizona Board of Regents on behalf of Arizona State University, Scottsdale, AZ (US)
Filed on Mar. 6, 2020, as Appl. No. 16/812,225.
Application 16/812,225 is a continuation of application No. 15/649,351, filed on Jul. 13, 2017, granted, now 10,669,534.
Claims priority of provisional application 62/361,884, filed on Jul. 13, 2016.
Prior Publication US 2020/0270599 A1, Aug. 27, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 11/04 (2006.01); B82Y 5/00 (2011.01); C12N 11/06 (2006.01); C12N 11/10 (2006.01); C12N 15/11 (2006.01)
CPC C12N 11/04 (2013.01) [C12N 11/06 (2013.01); C12N 11/10 (2013.01); C12N 15/11 (2013.01); B82Y 5/00 (2013.01); C12N 2310/532 (2013.01)] 20 Claims
 
1. A nanocage comprising;
a plurality of structural members comprising a single-stranded circular DNA sequence selected from p7308, p7560, p7704, p8064, p8634, and pEGFP, wherein internal surfaces of the plurality of structural members form an inner cavity which is defined by at least three pairs of opposite walls, wherein architectural arrangement of the structural members in the three dimensional body forms an arrangement selected from the group consisting of a honeycomb lattice, a single-walled square lattice, and a double-walled square lattice, and
wherein the at least three pairs of opposite walls comprises a first pair, a second pair, and a third pair of opposite walls, and
wherein each of the first pair of opposite walls comprises a thickness which is different from the other pairs of opposite walls.