	US 12,077,748 B2
	Methods and materials for producing recombinant viruses in eukaryotic microalgae
Brian K. Kaspar, Westerville, OH (US)
Assigned to The Research Institute at Nationwide Children's Hospital, Columbus, OH (US)
Filed by The Research Institute at Nationwide Children's Hospital, Columbus, OH (US)
Filed on Feb. 9, 2022, as Appl. No. 17/668,123.
Application 17/668,123 is a continuation of application No. 17/132,440, filed on Dec. 23, 2020, abandoned.
Application 17/132,440 is a continuation of application No. 15/524,963, granted, now 10,907,130, previously published as PCT/US2015/059272, filed on Nov. 5, 2015.
Claims priority of provisional application 62/075,408, filed on Nov. 5, 2014.
Prior Publication US 2022/0282205 A1, Sep. 8, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 1/12 (2006.01); C12N 7/00 (2006.01); C12N 15/86 (2006.01)

CPC C12N 1/12 (2013.01) [C12N 7/00 (2013.01); C12N 15/86 (2013.01); C12N 2750/14122 (2013.01); C12N 2750/14143 (2013.01); C12N 2750/14151 (2013.01); C12N 2800/22 (2013.01)]

22 Claims

1. A eukaryotic microalgae that produces a recombinant adeno-associated virus (rAAV), wherein the rAAV is a viral vaccine vector or a viral gene therapy vector, and the genome of the rAAV comprises inverted terminal repeats flanking a vaccine or gene therapy polynucleotide.