US 12,077,746 B2
Stable inoculant compositions and methods for producing same
Dave Greenshields, Sasketchewan (CA); Kristi Woods, Blacksburg, VA (US); Shaun Raj Selness, Chesterfield, MO (US); and Hui Han, Chesterfield, MO (US)
Assigned to NOVOZYMES BIOAG A/S, Bagsvaerd (DK); and MONSANTO TECHNOLOGY LLC., St. Louis, MO (US)
Filed by NOVOZYMES BIOAG A/S, Bagsvaerd (DK); and MONSANTO TECHNOLOGY LLC., St. Louis, MO (US)
Filed on Apr. 6, 2021, as Appl. No. 17/223,154.
Application 17/223,154 is a division of application No. 16/066,377, granted, now 11,076,603, previously published as PCT/US2016/067745, filed on Dec. 20, 2016.
Claims priority of provisional application 62/296,784, filed on Feb. 18, 2016.
Claims priority of provisional application 62/271,873, filed on Dec. 28, 2015.
Prior Publication US 2021/0219554 A1, Jul. 22, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 1/04 (2006.01); A01N 25/04 (2006.01); A01N 25/30 (2006.01); A01N 63/30 (2020.01); A01N 63/36 (2020.01); C12N 1/14 (2006.01); C12R 1/80 (2006.01)
CPC C12N 1/04 (2013.01) [A01N 25/04 (2013.01); A01N 25/30 (2013.01); A01N 63/30 (2020.01); A01N 63/36 (2020.01); C12N 1/14 (2013.01); C12N 1/145 (2021.05); A01N 2300/00 (2013.01); C12R 2001/80 (2021.05)] 20 Claims
 
1. An aqueous inoculant composition, comprising:
microbial spores comprising about 0.1 to about 15% by weight of said inoculant composition;
one or more protectants comprising about 0.5 to about 5% by weight of said composition, said one or more protectants comprising one or more sugars and/or one or more sugar alcohols;
one or more dispersants comprising about 0.1 to about 5% by weight of said composition;
one or more aqueous additives comprising about 0.01 to about 5% by weight of said composition; and
one or more polyethylene glycols comprising about 75 to about 95% by weight of said inoculant composition.