US 12,077,742 B2
Methods for fluid mixing systems with laterally displaced flexible drive lines
Nephi D. Jones, Newton, UT (US); and Christopher D. Brau, Logan, UT (US)
Assigned to Life Technologies Corporation, Carlsbad, CA (US)
Filed by Life Technologies Corporation, Carlsbad, CA (US)
Filed on Apr. 21, 2020, as Appl. No. 16/853,865.
Application 16/853,865 is a division of application No. 15/392,906, filed on Dec. 28, 2016, granted, now 10,669,515.
Claims priority of provisional application 62/272,504, filed on Dec. 29, 2015.
Prior Publication US 2020/0248125 A1, Aug. 6, 2020
Int. Cl. C12M 1/06 (2006.01); B01F 27/1111 (2022.01); B01F 27/191 (2022.01); B01F 27/2111 (2022.01); B01F 35/41 (2022.01); B01F 35/513 (2022.01); B01F 101/44 (2022.01); C12M 1/00 (2006.01); C12M 1/02 (2006.01); C12N 1/00 (2006.01)
CPC C12M 27/02 (2013.01) [B01F 27/1111 (2022.01); B01F 27/191 (2022.01); B01F 27/2111 (2022.01); B01F 35/4121 (2022.01); B01F 35/513 (2022.01); C12M 1/007 (2013.01); C12M 1/02 (2013.01); C12M 23/14 (2013.01); C12N 1/00 (2013.01); B01F 2101/44 (2022.01)] 7 Claims
OG exemplary drawing
 
1. A method for mixing a biological suspension, the method comprising:
disposing a biological suspension within a compartment of a container, the biological suspension comprising cells or microorganisms suspended within a nutrient growth medium; and
rotating a first drive line and laterally spaced apart second drive line within the compartment of the container so as to cause the drive lines to twist around each other to thereby transition from a non-helical configuration into a helical configuration and mix the biological suspension, wherein the drive lines are configured to transition between the non-helical configuration and the helical configuration by twisting and untwisting, wherein the non-helical configuration comprises the first drive line and the second drive line arranged parallel to each other, and the helical configuration comprises the first drive line and second drive line arranged around each other in a double helix.