US 12,077,612 B2
Peptide compositions
Shubh Sharma, Cranbury, NJ (US); Leonardus H. T. Van Der Ploeg, Newton, MA (US); and Bart Henderson, Belmont, MA (US)
Assigned to RHYTHM PHARMACEUTICALS, INC., Boston, MA (US)
Filed by RHYTHM PHARMACEUTICALS, INC., Boston, MA (US)
Filed on Oct. 26, 2020, as Appl. No. 17/080,293.
Application 16/216,116 is a division of application No. 14/775,916, granted, now 10,196,425, issued on Feb. 5, 2019, previously published as PCT/US2014/028590, filed on Mar. 14, 2014.
Application 17/080,293 is a continuation of application No. 16/216,116, filed on Dec. 11, 2018, granted, now 10,858,399.
Claims priority of provisional application 61/790,469, filed on Mar. 15, 2013.
Prior Publication US 2021/0040155 A1, Feb. 11, 2021
Int. Cl. C07K 7/54 (2006.01); A61K 38/00 (2006.01); A61K 38/12 (2006.01); C07K 14/685 (2006.01)
CPC C07K 7/54 (2013.01) [A61K 38/12 (2013.01); C07K 14/685 (2013.01); A61K 38/00 (2013.01)] 6 Claims
 
1. An isolated polypeptide of the following structural Formula (I):

OG Complex Work Unit Chemistry
or a pharmaceutically acceptable salt thereof,
wherein:
R1 is —H, or a C1-C6 acyl;
R2 is —NR3R4, or —OR5 wherein R3, R4, and R5 are each independently is-H or a C1-C6 alkyl;
A1 Nle;
A2 and A8 is each independently an amino acid residue selected from Cys, hCys, Glu, or Dpr, wherein A2 and A8 are pairwise selected so as to be able to form a covalent bond between their respective side chains;
A3 is an amino acid residue selected from Ala, Tle, Val, Leu, Ile, Cha, Pro, Ser, Thr, Lys, Arg, His, Phe, Gln, Sar, Gly, Asn, Aib, or
A3 is absent, or
A3 is residue Y, wherein Y is an amino acid selected from amino acids represented by the following structural formulas

OG Complex Work Unit Chemistry
wherein:
R11 and R12, each independently, is H, —CH3, phenyl, or benzyl;
R21, R22, R23, and R24, each independently is H, —CH3, —CF3, phenyl, benzyl, F, Cl, Br, I, —OCH3, or —OH;
R31, R32, R33, R34, R41, R42, and R43, each independently is H, —CH3, —CF3, phenyl, benzyl, F, Cl, Br, I, —OCH3, or —OH;
A4 is absent or an optionally substituted His;
A5 is an optionally substituted Phe
A6 is Arg; and
A7 is Trp,
wherein any amino acid residue is either in L- or in D-configuration.