US 12,077,592 B2
GPRC5D chimeric antigen receptors and cells expressing the same
Ricardo Attar, Lawrenceville, NJ (US); Rajkumar Ganesan, Blue Bell, PA (US); Francois Gaudet, Princeton, NJ (US); Bradley J. Heidrich, Gilbertsville, PA (US); Carmen Baca Jones, Ambler, PA (US); John Lee, North Wales, PA (US); Yingzhe Li, Dresher, PA (US); Sanjaya Singh, Blue Bell, PA (US); and Sathya Venkataramani, Blue Bell, PA (US)
Assigned to Janssen Biotech, Inc., Horsham, PA (US)
Filed by Janssen Biotech, Inc., Horsham, PA (US)
Filed on Jan. 15, 2020, as Appl. No. 16/743,188.
Claims priority of provisional application 62/793,973, filed on Jan. 18, 2019.
Prior Publication US 2020/0231686 A1, Jul. 23, 2020
Int. Cl. C07K 16/28 (2006.01); A61K 9/00 (2006.01); A61P 35/00 (2006.01); A61K 39/00 (2006.01)
CPC C07K 16/2866 (2013.01) [A61K 9/0019 (2013.01); A61P 35/00 (2018.01); A61K 2039/505 (2013.01); A61K 2039/54 (2013.01); A61K 2039/545 (2013.01); C07K 2317/24 (2013.01); C07K 2317/53 (2013.01); C07K 2317/565 (2013.01); C07K 2317/622 (2013.01); C07K 2317/73 (2013.01); C07K 2317/74 (2013.01); C07K 2319/02 (2013.01); C07K 2319/03 (2013.01); C07K 2319/30 (2013.01); C07K 2319/33 (2013.01)] 36 Claims
 
1. A chimeric antigen receptor (CAR), comprising an extracellular antigen-binding domain, a transmembrane domain and an intracellular signaling domain, wherein the extracellular antigen-binding domain comprises:
a heavy chain CDR1 having the amino acid sequence of SEQ ID NO: 66, a heavy chain CDR2 having the amino acid sequence of SEQ ID NO: 67, and a heavy chain CDR3 having the amino acid sequence of SEQ ID NO: 68, and further comprises a light chain CDR1 having the amino acid sequence of SEQ ID NO: 69, a light chain CDR2 having the amino acid sequence of SEQ ID NO: 70, and a light chain CDR3 having the amino acid sequence of SEQ ID NO: 71; or
a heavy chain CDR1 having the amino acid sequence of SEQ ID NO: 58, a heavy chain CDR2 having the amino acid sequence of SEQ ID NO: 59, and a heavy chain CDR3 having the amino acid sequence of SEQ ID NO: 60, and further comprises a light chain CDR1 having the amino acid sequence of SEQ ID NO: 61, a light chain CDR2 having the amino acid sequence of SEQ ID NO: 62, and a light chain CDR3 having the amino acid sequence of SEQ ID NO: 63;
wherein the extracellular antigen-binding domain binds the anti-G protein receptor family C group 5 member D (GPRC5D) antigen.