US 12,077,577 B2
Polypeptide comprising aggrecan binding domain and carrying moiety
Hidetomo Kitamura, Gotemba (JP); Maiko Hoshino, Singapore (SG); Yang Sun, Singapore (SG); Taichi Kuramochi, Singapore (SG); Wenjie Tu, Gotemba (JP); Tomoyuki Igawa, Singapore (SG); Naoka Hironiwa, Singapore (SG); and Yuki Noguchi, Gotemba (JP)
Assigned to Chugai Seiyaku Kabushiki Kaisha, Tokyo (JP)
Appl. No. 17/058,961
Filed by Chugai Seiyaku Kabushiki Kaisha, Tokyo (JP)
PCT Filed May 30, 2019, PCT No. PCT/JP2019/021463
§ 371(c)(1), (2) Date Nov. 25, 2020,
PCT Pub. No. WO2019/230867, PCT Pub. Date Dec. 5, 2019.
Claims priority of application No. 2018-103233 (JP), filed on May 30, 2018.
Prior Publication US 2021/0221875 A1, Jul. 22, 2021
Int. Cl. C07K 16/18 (2006.01); A61K 39/00 (2006.01)
CPC C07K 16/18 (2013.01) [A61K 2039/505 (2013.01); C07K 2317/56 (2013.01)] 27 Claims
OG exemplary drawing
 
1. A polypeptide comprising an aggrecan-binding domain linked by a peptide linker to a carrying moiety, wherein
(i) the aggrecan-binding domain has a molecular weight of 120 kDa or smaller and comprises the complementarity determining regions of:
(a) a VH sequence of SEQ ID NO: 512 and VL sequence of SEQ ID NO: 513,
(b) a VH sequence of SEQ ID NO: 514 and VL sequence of SEQ ID NO: 515,
(c) a VH sequence of SEQ ID NO: 516 and VL sequence of SEQ ID NO: 517, or
(d) a VH sequence of SEQ ID NO: 510 and VL sequence of SEQ ID NO: 511,
(ii) the peptide linker comprises a protease cleavage site, and
(iii) the carrying moiety comprises an antibody constant region.