US 12,077,566 B2
Mutants of recombinant immunoregulatory protein of Ganoderma lucidum and applications thereof
Fei Sun, Jilin (CN); and Chongyang Liang, Jilin (CN)
Assigned to Xitian Zhang, Jilin (CN); Fei Sun, Jilin (CN); and Zin Zhang, Jilin (CN)
Appl. No. 16/462,941
Filed by Xitian Zhang, Jilin (CN); Fei Sun, Jilin (CN); and Xin Zhang, Jilin (CN)
PCT Filed Mar. 14, 2018, PCT No. PCT/CN2018/078934
§ 371(c)(1), (2) Date May 22, 2019,
PCT Pub. No. WO2018/184448, PCT Pub. Date Oct. 11, 2018.
Claims priority of application No. 201710226093.3 (CN), filed on Apr. 8, 2017.
Prior Publication US 2019/0352345 A1, Nov. 21, 2019
Int. Cl. C07K 14/375 (2006.01); A61K 38/00 (2006.01); A61K 38/17 (2006.01); A61P 35/00 (2006.01); A61P 35/04 (2006.01)
CPC C07K 14/375 (2013.01) [A61P 35/00 (2018.01); A61P 35/04 (2018.01); A61K 38/00 (2013.01); A61K 38/1754 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A Lingzhi-8 (LZ-8; SEQ ID NO: 1) mutant, wherein the LZ-8 mutant comprises a mutation selected from the group consisting of:
(1) the amino acid D at position 70 is replaced with K (D70K);
(2) the amino acid K at position 46 is replaced with E (K46E);
(3) the amino acid L at position 17 is replaced with K and the amino acid D at position 70 is replaced with K (L17K and D70K);
(4) the amino acid D at position 20 is replaced with H (D20H);
(5) the amino acid L at position 17 is replaced with K (L17K);
(6) the amino acid K at position 46 is replaced with E and the amino acid K at position 74 is replaced with E (K46E and K74E); and
(7) the amino acid D at position 20 is replaced with H and the amino acid D at position 70 is replaced with K (D20H and D70K).