	US 12,077,565 B2
	Claudin-targeting agents and uses thereof
Enrique Gamero-Estevez, Montreal (CA); Aimee Ryan, Montreal (CA); and Makoto Nagano, Dorval (CA)
Filed by Enrique Gamero-Estevez, Montreal (CA); Aimee Ryan, Montreal (CA); and Makoto Nagano, Dorval (CA)
Filed on Oct. 19, 2020, as Appl. No. 17/073,817.
Claims priority of provisional application 62/923,481, filed on Oct. 19, 2019.
Prior Publication US 2021/0130418 A1, May 6, 2021
Int. Cl. C07K 14/33 (2006.01)

CPC C07K 14/33 (2013.01) [C07K 2319/21 (2013.01); C07K 2319/23 (2013.01); C07K 2319/50 (2013.01)]

12 Claims

1. A polypeptide construct consisting of:

a first region comprising a C-terminal domain of Clostridium perfringens enterotoxin (C-CPE) with the claudin-binding domain removed; and

a second region comprising the second extracellular loop (EL2) of a claudin protein;

wherein the polypeptide construct specifically targets one or more claudin protein in the blood-brain barrier, the intestinal barrier, the blood-testis barrier (BTB), or the kidney nephronic barrier and can transiently disrupt tight junctions therein.