US 12,077,557 B2
Puromycin-based probes and methods of use thereof
Christopher J. Chang, Berkeley, CA (US); and Yik Sham Clive Chung, Berkeley, CA (US)
Assigned to The Regents of the University of California, Oakland, CA (US)
Appl. No. 16/981,511
Filed by The Regents of the University of California, Oakland, CA (US)
PCT Filed Mar. 20, 2019, PCT No. PCT/US2019/023242
§ 371(c)(1), (2) Date Sep. 16, 2020,
PCT Pub. No. WO2019/183270, PCT Pub. Date Sep. 26, 2019.
Claims priority of provisional application 62/647,481, filed on Mar. 23, 2018.
Prior Publication US 2021/0061842 A1, Mar. 4, 2021
Int. Cl. C07H 19/16 (2006.01); C12Q 1/26 (2006.01); G01N 33/58 (2006.01)
CPC C07H 19/16 (2013.01) [C12Q 1/26 (2013.01); G01N 33/582 (2013.01)] 18 Claims
 
1. A compound of formula (I):
P-L-T  (I)
wherein:
P is a puromycin detectable moiety;
L is a self-immolative linker; and
T is an analyte-responsive group configured to trigger cleavage of L and release P upon contact with a target analyte,
wherein the puromycin detectable moiety is:

OG Complex Work Unit Chemistry