US 12,077,473 B1
Inorganic solid waste-microbial composite curing agent and its preparation method and application
Anhui Wang, Nanjing (CN); Zhanwei Huang, Nanjing (CN); Yanfang Zhang, Nanjing (CN); Jiaojiao Ni, Nanjing (CN); Rui Zhang, Nanjing (CN); and Jiao Lin, Nanjing (CN)
Assigned to CHINA CONSTRUCTION INDUSTRIAL & ENERGY ENGINEERING GROUP CO., LTD.
Filed by CHINA CONSTRUCTION INDUSTRIAL & ENERGY ENGINEERING GROUP CO., LTD., Nanjing (CN)
Filed on Mar. 13, 2024, as Appl. No. 18/603,602.
Claims priority of application No. 202310242854.X (CN), filed on Mar. 14, 2023.
Int. Cl. C04B 20/02 (2006.01); C04B 40/00 (2006.01); C04B 40/02 (2006.01); C04B 111/00 (2006.01); C12N 1/20 (2006.01); C12R 1/125 (2006.01)
CPC C04B 20/023 (2013.01) [C04B 40/0042 (2013.01); C04B 40/0231 (2013.01); C12N 1/205 (2021.05); C04B 2111/00767 (2013.01); C12R 2001/125 (2021.05)] 7 Claims
 
1. An inorganic solid waste-microbial composite curing agent, characterized in that: the inorganic solid waste-microbial composite curing agent comprises following components in parts by weight: 20-30 parts of steel slag, 30-40 parts of activated magnesium oxide, 20-30 parts of recycled powder, 5-10 parts of foaming agent, 1-5 parts of foam stabilizer, 0.4-0.8 parts of composite microbial agent, 2-8 parts of soluble calcium salt; the composite microbial agent comprises carbonic anhydrase bacteria microcapsules and Bacillus subtilis microcapsules,
produced by a method comprising the following steps:
S1. preparation of solid waste porous material:
mixing the steel slag, the activated magnesium oxide, the recycled powder, the foaming agent, the foam stabilizer and performing extrusion granulation, heating the material to 1110-1120° C., then cutting, granulating, and sieving to obtain a solid waste porous material;
S2. modification of the solid waste porous material:
taking zinc nitrate hexahydrate and ferrous chloride to add into a mixed solution of methanol and deionized water, then adding the solid waste porous material, stirring for 2-3 h, then washing and drying to obtain a modified solid waste porous material;
S3. cultivation of carbonic anhydrase bacteria and Bacillus subtilis with high pH resistance:
conducting domestication on carbonic anhydrase bacteria and Bacillus subtilis separately, while adjusting a pH of a culture solution to 10-12, and screening the carbonic anhydrase bacteria and the Bacillus subtilis to obtain the carbonic anhydrase bacteria and Bacillus subtilis that are able to tolerate a pH of 10;
S4. preparation of the composite bacterial agent of carbonic anhydrase bacteria and Bacillus subtilis:
performing centrifugation to obtain carbonic anhydrase bacterial sludge and Bacillus subtilis bacterial sludge respectively, then separately adding sodium alginate saturated solution, then performing centrifugation after stirring to obtain carbonic anhydrase bacteria microcapsules and Bacillus subtilis microcapsules, respectively, then mixing the carbonic anhydrase bacteria microcapsules and the Bacillus subtilis microcapsules uniformly, and then drying to obtain the composite microbial agent:
S5. preparation of the inorganic solid waste-microbial composite curing agent
adding the composite microbial agent and the soluble calcium salt into deionized water to obtain a mixed solution, then impregnating the modified solid waste porous material in the mixed solution for 15-20 min under vacuum negative pressure, then drying to obtain the inorganic solid waste-microbial composite curing agent.