US 12,076,701 B2
Capturing oligonucleotides in spatial transcriptomics
Felice Alessio Bava, Pleasanton, CA (US)
Assigned to 10x Genomics, Inc., Pleasanton, CA (US)
Filed by 10X Genomics, Inc., Pleasanton, CA (US)
Filed on Jan. 28, 2021, as Appl. No. 17/161,007.
Claims priority of provisional application 62/985,281, filed on Mar. 4, 2020.
Claims priority of provisional application 62/968,312, filed on Jan. 31, 2020.
Prior Publication US 2021/0237022 A1, Aug. 5, 2021
Int. Cl. B01J 19/00 (2006.01)
CPC B01J 19/0046 (2013.01) [B01J 2219/00608 (2013.01); B01J 2219/00722 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A composition, comprising:
a nucleic acid array comprising a support having a plurality of first probes affixed to the support, wherein each of the first probes comprises 2, 3, 4, or 5 copies of a first nucleotide hybridization sequence; and
a plurality of second probes, wherein each of the second probes comprises (i) a second hybridization sequence complementary to the first nucleotide hybridization sequence, and (ii) a unique molecular identifier;
wherein:
two or more of the copies of the first nucleotide hybridization sequence in each of the first probes are hybridized to the second nucleotide hybridization sequence of the second probes;
each of the first probes and each of the second probes comprises a unique spatial barcode associated with a unique location on the nucleic acid array; and
the unique spatial barcode of each of the first probes and each of the second probes does not substantially hybridize to a nucleic acid in a biological sample.