	US 12,076,434 B2
	Method of production of a plant cell extract of hydroxyproline rich glycoproteins including extensins
Chiara Marzotto, Mortara (IT); and Roberto Dal Toso, Mortara (IT)
Assigned to Croda Italiana S.P.A., Mortara (IT)
Appl. No. 17/253,423
Filed by Croda Italiana S.P.A, Mortara (IT)
PCT Filed Jul. 4, 2019, PCT No. PCT/EP2019/067943 § 371(c)(1), (2) Date Dec. 17, 2020, PCT Pub. No. WO2020/007959, PCT Pub. Date Jan. 9, 2020.
Claims priority of application No. 102018000006983 (IT), filed on Jul. 6, 2018.
Prior Publication US 2021/0169775 A1, Jun. 10, 2021
Int. Cl. A61K 8/9789 (2017.01); A61K 8/64 (2006.01); A61K 8/97 (2017.01); A61Q 19/08 (2006.01)

CPC A61K 8/9789 (2017.08) [A61K 8/64 (2013.01); A61K 8/645 (2013.01); A61K 8/97 (2013.01); A61Q 19/08 (2013.01)]

13 Claims

1. A method for producing a plant cell extract of monomeric hydroxyl rich glycoproteins (HRGP) by extraction from plant cell walls (PCW), the method comprising subjecting a biomass of dedifferentiated and/or meristematic plant cells prepared by in-vitro cell culture to the following treatment:

A) breaking whole cells for producing a cell homogenate in which the intracellular molecules are solubilized;

B) recovering the PCW by eliminating the solubilized intracellular molecules by washing the cell homogenate with an aqueous solution;

C) extracting the HRGP from the PCW by adding a salt thereby forming an HRGP enriched salted extract suspension; and

D) recovering the HRGP enriched salted extract suspension by discarding the PCW debris,

wherein the HRGP include extensins.