	US 12,076,416 B2
	Gene editing nanocapsule and preparation method and use thereof
Yan Zou, Kaifeng (CN); Bingyang Shi, Kaifeng (CN); Meng Zheng, Kaifeng (CN); and Xinhong Sun, Kaifeng (CN)
Assigned to Henan University, Kaifeng (CN)
Filed by Henan University, Kaifeng (CN)
Filed on Feb. 18, 2022, as Appl. No. 17/675,556.
Claims priority of application No. 202110260752.1 (CN), filed on Mar. 10, 2021.
Prior Publication US 2022/0323605 A1, Oct. 13, 2022
Int. Cl. A61K 47/69 (2017.01); A61K 38/46 (2006.01); A61P 35/00 (2006.01); C12N 11/04 (2006.01); C12N 15/11 (2006.01)

CPC A61K 47/6925 (2017.08) [A61K 38/465 (2013.01); A61P 35/00 (2018.01); C12N 15/11 (2013.01); C12N 11/04 (2013.01); C12N 2310/20 (2017.05); C12N 2310/351 (2013.01); C12N 2310/3513 (2013.01)]

17 Claims

1. A gene editing nanocapsule, comprising an inner core and an outer shell encapsulating the inner core, wherein the inner core comprises a Cas/sgRNA ribonucleoprotein complex obtained by combining Cas nuclease and sgRNA, wherein the Cas nuclease is Cas9 nuclease, and a molar ratio of the Cas nuclease and the sqRNA is 1:1.2; and the outer shell comprises a polymer polymerized by a monomer material, wherein the monomer material comprises a first monomer and a second monomer that can be polymerized capable of polymerizing with each other, wherein the first monomer is guanidino acrylate and the second monomer is N,N′-bis(acryloyl)cystamine, and a molar ratio of the Cas nuclease and the guanidino acrylate is 1:200-250.