US 12,076,394 B2
Cytosolic delivery of peptides using protein transduction domain mimics
Coralie M. Backlund, Amherst, MA (US); and Gregory N. Tew, South Deerfield, MA (US)
Assigned to UNIVERSITY OF MASSACHUSETTS, Boston, MA (US)
Filed by University of Massachusetts, Boston, MA (US)
Filed on May 14, 2021, as Appl. No. 17/321,168.
Claims priority of provisional application 63/025,210, filed on May 15, 2020.
Prior Publication US 2021/0353746 A1, Nov. 18, 2021
Int. Cl. A61K 39/385 (2006.01); A61K 39/00 (2006.01); A61K 47/32 (2006.01); C08F 234/02 (2006.01); C08G 61/00 (2006.01)
CPC A61K 39/385 (2013.01) [A61K 47/32 (2013.01); C08F 234/02 (2013.01); A61K 2039/55561 (2013.01); A61K 2039/6093 (2013.01); C08G 61/00 (2013.01); C08G 2261/126 (2013.01); C08G 2261/1426 (2013.01); C08G 2261/143 (2013.01)] 10 Claims
 
1. A complex of a peptide with one or more polymer molecules, wherein the peptide is an antigen comprising about 3 to about 50 amino acid residues and is non-covalently complexed to one or more molecules of a block copolymer having the Formula of (I):

OG Complex Work Unit Chemistry
wherein
X1, X2 each is independently O or CH2;
Y11, Y12 each is independently a single bond or a linking group;
Z11, Z12 each is independently

OG Complex Work Unit Chemistry
wherein each R is independently hydrogen or a C1-C6 alkyl group;
Y21, Y22 each is independently a single bond or a linking group;
Z21, Z22 each is independently alkyl, substituted alkyl, aryl, or substituted aryl group; and
m, n each is independently an integer from about 2 to about 50,
wherin the complex further comprises a TLR9 agonist CpG and is non-covalently complexed to the one or more molecules of a block copolymer having the Formula (I).