	US 12,076,391 B2
	Method for adapting influenza viruses to Vero cells
Yi Zhang, Singapore (SG); Yuhe Yan, Singapore (SG); Xu Zhang, Singapore (SG); and Thomas Anthony Coton, Singapore (SG)
Assigned to Yisheng Biopharma (Singapore) Pte Ltd, Singapore (SG)
Filed by YISHENG BIOPHARMA (SINGAPORE) PTE. LTD., Singapore (SG)
Filed on Mar. 2, 2023, as Appl. No. 18/177,413.
Application 18/177,413 is a continuation of application No. 16/960,283, granted, now 11,612,648, previously published as PCT/SG2018/050030, filed on Jan. 18, 2018.
Prior Publication US 2023/0310578 A1, Oct. 5, 2023
Int. Cl. A61K 39/145 (2006.01); A61K 39/00 (2006.01); A61K 39/12 (2006.01); C12N 5/00 (2006.01); C12N 7/00 (2006.01)

CPC A61K 39/145 (2013.01) [C12N 7/00 (2013.01); C12N 2760/16152 (2013.01)]

20 Claims

1. A method for adapting an influenza virus to Vero cells, comprising:

infecting Vero cells with an influenza virus at a first infectious dose;

cultivating infected Vero cells in a spinner flask to produce viral activities;

harvesting a first influenza virus;

infecting Vero cells with said first influenza virus at a second infectious dose to produce viral activities, wherein said second infectious dose is greater than or equal to said first infectious dose;

harvesting a second influenza virus; and

repeating the process, and harvesting a Vero cell adapted influenza virus,

wherein the Vero cells are attached to microcarriers.