	US 12,076,377 B2
	Variant sulfatase enzymes with enhanced properties
Lim Andrew Lee, Columbia, SC (US); Caleb Reece Schlachter, Irmo, SC (US); and John Tomashek, Columbia, SC (US)
Assigned to INTEGRATED MICRO-CHROMATOGRAPHY SYSTEMS, INC., Irmo, SC (US)
Filed by INTEGRATED MICRO-CHROMATOGRAPHY SYSTEMS, INC., Irmo, SC (US)
Filed on Oct. 26, 2022, as Appl. No. 18/049,978.
Claims priority of provisional application 63/271,936, filed on Oct. 26, 2021.
Prior Publication US 2023/0149519 A1, May 18, 2023
Int. Cl. A61K 38/46 (2006.01); A61K 38/16 (2006.01); C07K 14/00 (2006.01); C12N 9/16 (2006.01)

CPC A61K 38/465 (2013.01) [A61K 38/16 (2013.01); C07K 14/00 (2013.01); C12N 9/16 (2013.01); C12Y 301/06013 (2013.01)]

9 Claims

1. A variant Pseudomonas aeruginosa sulfatase (PaS) enzyme comprising an amino acid substitution at an amino acid position corresponding to A139, of a parental PaS enzyme comprising the amino acid sequence shown in SEQ ID NO: 1.