	US 12,076,351 B2
	AAV vectors targeted to oligodendrocytes
Thomas McCown, Carrboro, NC (US); and Steven Gray, Southlake, TX (US)
Assigned to The University of North Carolina at Chapel Hill, Chapel Hill, NC (US)
Filed by The University of North Carolina at Chapel Hill, Chapel Hill, NC (US)
Filed on Jul. 15, 2019, as Appl. No. 16/511,913.
Application 16/511,913 is a division of application No. 15/488,966, filed on Apr. 17, 2017, abandoned.
Application 15/488,966 is a division of application No. 14/431,900, granted, now 9,636,370, issued on May 2, 2017, previously published as PCT/US2013/062240, filed on Sep. 27, 2013.
Claims priority of provisional application 61/707,108, filed on Sep. 28, 2012.
Prior Publication US 2019/0328804 A1, Oct. 31, 2019
Int. Cl. C12N 15/86 (2006.01); A61K 9/00 (2006.01); A61K 35/76 (2015.01); C07K 14/005 (2006.01); C12N 7/00 (2006.01); A61K 39/23 (2006.01); C07K 14/015 (2006.01); C12N 15/864 (2006.01)

CPC A61K 35/76 (2013.01) [A61K 9/0085 (2013.01); C07K 14/005 (2013.01); C12N 7/00 (2013.01); C12N 15/86 (2013.01); A61K 39/23 (2013.01); C07K 14/015 (2013.01); C12N 15/8645 (2013.01); C12N 2750/14122 (2013.01); C12N 2750/14142 (2013.01); C12N 2750/14143 (2013.01); C12N 2750/14145 (2013.01); C12N 2810/6027 (2013.01)]

23 Claims

1. A method of delivering a nucleic acid of interest to an oligodendrocyte, the method comprising directly contacting the oligodendrocyte with an adeno-associated virus (AAV) particle comprising:

an AAV vector genome comprising or encoding the nucleic acid of interest encapsidated by—an AAV capsid comprising an amino acid sequence at least 96% identical to SEQ ID NOS: 2 or 4, that exhibits a preferential tropism for oligodendrocytes as compared to astrocytes, microglia and neurons.