US 12,075,786 B2
Markers of plant health
Barry S. Goldman, Cambridge, MA (US); Max E. Winston, Somerville, MA (US); and Allison Perrotta, Somerville, MA (US)
Assigned to Indigo Ag, Inc., Boston, MA (US)
Filed by Indigo Ag, Inc., Boston, MA (US)
Filed on Sep. 18, 2018, as Appl. No. 16/134,720.
Claims priority of provisional application 62/651,033, filed on Mar. 30, 2018.
Claims priority of provisional application 62/560,124, filed on Sep. 18, 2017.
Prior Publication US 2019/0085352 A1, Mar. 21, 2019
This patent is subject to a terminal disclaimer.
Int. Cl. A01N 63/27 (2020.01); A01H 3/00 (2006.01); A01H 17/00 (2006.01); A01N 63/20 (2020.01); A01N 63/22 (2020.01); A01N 63/25 (2020.01); A01N 63/28 (2020.01); C12N 1/20 (2006.01); C12N 15/10 (2006.01); C12N 15/82 (2006.01); C12Q 1/06 (2006.01); C12Q 1/689 (2018.01); C12Q 1/6895 (2018.01)
CPC A01N 63/27 (2020.01) [A01H 3/00 (2013.01); A01H 17/00 (2013.01); A01N 63/20 (2020.01); A01N 63/22 (2020.01); A01N 63/25 (2020.01); A01N 63/28 (2020.01); C12N 1/20 (2013.01); C12N 15/1068 (2013.01); C12N 15/1075 (2013.01); C12N 15/1079 (2013.01); C12N 15/8261 (2013.01); C12Q 1/06 (2013.01); C12Q 1/689 (2013.01); C12Q 1/6895 (2013.01)] 12 Claims
 
1. A synthetic composition comprising a plant element and an endophyte that is heterologously disposed to the plant element, wherein said endophyte comprises:
i) a nucleic acid sequence that is at least 99% identical to the nucleic acid sequence shown in SEQ ID NO 198, wherein said endophyte is disposed in an amount effective to improve plant health as compared to a reference plant element not further comprising said endophyte;
ii) a nucleic acid sequence that is at least 99% identical to the nucleic acid sequence shown in SEQ ID NO 198, wherein said endophyte is disposed in an amount effective to enrich the abundance of Alphaproteobacteria are relative to Gammaproteobacteria as compared to a reference plant element not further comprising said endophyte;
iii) a nucleic acid sequence that is at least 99% identical to the nucleic acid sequence shown in SEQ ID NO 49, wherein said endophyte is disposed in an amount effective to improve plant health as compared to a reference plant element not further comprising said endophyte;
iv) a nucleic acid sequence that is 100% identical to the nucleic acid sequence shown in SEQ ID NO 230 wherein said endophyte is disposed in an amount effective to improve plant health as compared to a reference plant element not further comprising said endophyte;
v) a nucleic acid sequence that is at least 99% identical to the nucleic acid sequence shown in SEQ ID NO 317, wherein said endophyte is disposed in an amount effective to improve plant health as compared to a reference plant element not further comprising said endophyte;
vi) a nucleic acid sequence that is at least 99% identical to the nucleic acid sequence shown in SEQ ID NO 51, wherein the endophyte is heterologously disposed to the plant element in an amount effective to increase the taxonomic diversity of the microbial community; or
vii) a nucleic acid sequence that is at least 99% identical to the nucleic acid sequence shown in SEQ ID NO 198, wherein the endophyte treatment is heterologously disposed to the plant element in an amount effective to increase the abundance in the plant element or plant grown from the plant element, of one or more endophytes comprising a nucleic acid sequence that is at least 99% identical to a nucleic acid sequence selected from the group consisting of SEQ ID NOs. 198, 49, and 230, wherein the one or more endophytes that increase in abundance were not heterologously disposed to the plant element.