| CPC G06T 7/0012 (2013.01) [A61B 5/4514 (2013.01); G06T 2207/30008 (2013.01)] | 21 Claims |

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19. A non-transitory computer-readable medium, the computer-readable medium comprising processor-executable code that when executed by a processor, causes the processor to:
pre-process a segmented image of region of interests (ROIs) of a knee of a subject to separate the ROIs into individual ROI volumes, wherein the ROI volumes comprise at least one cartilage region;
perform surface separation between a respective subchondral surface and a respective articular surface for each ROI volume to extract a respective separate subchondral surface and a respective separate articular surface for each ROI volume; and
estimate cartilage thickness values for the at least one cartilage region utilizing a nearest neighbor algorithm based on the respective separate subchondral surface and the respective separate articular surface for each ROI volume, wherein pre-processing the segmented image, performing surface separation, and estimating cartilage thickness values all occur without utilizing deep-learning.
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