	US 12,405,277 B2
	Hemoglobin assay reagent, assay kit and assay method
Megumi Yui, Tochigi (JP); and Mitsuru Makinodan, Tochigi (JP)
Assigned to EIKEN KAGAKU KABUSHIKI KAISHA, Tokyo (JP)
Appl. No. 17/278,647
Filed by EIKEN KAGAKU KABUSHIKI KAISHA, Tokyo (JP)
PCT Filed Sep. 13, 2019, PCT No. PCT/JP2019/036235 § 371(c)(1), (2) Date Mar. 22, 2021, PCT Pub. No. WO2020/066722, PCT Pub. Date Apr. 2, 2020.
Claims priority of application No. 2018-179952 (JP), filed on Sep. 26, 2018.
Prior Publication US 2022/0043008 A1, Feb. 10, 2022
Int. Cl. G01N 33/72 (2006.01); G01N 33/545 (2006.01); G01N 33/553 (2006.01)

CPC G01N 33/721 (2013.01) [G01N 33/545 (2013.01); G01N 33/553 (2013.01)]

15 Claims

5. A hemoglobin measurement method for measuring a total amount of hemoglobin in a specimen, wherein the total amount of hemoglobin is the total amount of a hemoglobin-haptoglobin complex and free hemoglobin, the method comprising:

a step (1) of mixing a specimen with haptoglobin and forming a hemoglobin-haptoglobin complex to obtain a sample containing the hemoglobin-haptoglobin complex;

a step (2) of mixing the sample obtained in the step (1) with a first insoluble carrier immobilizing an anti-hemoglobin antibody and a second insoluble carrier immobilizing an anti-haptoglobin antibody; and

a step (3) of measuring the total amount of hemoglobin-haptoglobin complex and the free hemoglobin.