US 12,405,272 B2
Assay device
Catherine Ungeprapakorn, Geneva (CH); Stephen Carlisle, Geneva (CH); and Balbir Raj, Geneva (CH)
Assigned to SPD Swiss Precision Diagnostics GMBH, Geneva (CH)
Appl. No. 17/602,592
Filed by SPD Swiss Precision Diagnostics GMBH, Geneva (CH)
PCT Filed Apr. 9, 2020, PCT No. PCT/EP2020/060155
§ 371(c)(1), (2) Date Oct. 8, 2021,
PCT Pub. No. WO2020/208141, PCT Pub. Date Oct. 15, 2020.
Claims priority of application No. 1905090 (GB), filed on Apr. 10, 2019.
Prior Publication US 2022/0187325 A1, Jun. 16, 2022
Int. Cl. G01N 33/543 (2006.01); G01N 33/76 (2006.01)
CPC G01N 33/54388 (2021.08) [G01N 33/54387 (2021.08); G01N 33/76 (2013.01); G01N 2333/59 (2013.01)] 25 Claims
OG exemplary drawing
 
1. An assay device for detecting the presence of an analyte in a sample, the device comprising an assay flow path, the flow path comprising:
(a) a reagent zone comprising a mobilisable labelled conjugate comprising a detectable label directly or indirectly linked to
means for associating the labelled conjugate with the analyte and means for associating the labelled conjugate with a first negative marker which may be present in the sample;
(b) one or more capture zones, wherein
at least one capture zone comprises an immobilised capture species configured to capture a complex comprising the labelled conjugate and the first negative marker wherein capture of the complex is achieved by a specific binding reaction with first negative marker; and
(c) a detection zone comprising immobilised binding reagent for capturing a complex comprising the labelled conjugate and the analyte, wherein the one or more capture zones is downstream of the reagent zone, and the detection zone is downstream of the one or more capture zones.