US 12,405,271 B2
Method and apparatus for bacterial analysis
Massimo Antognozzi, Bristol (GB); Charlotte Bermingham, Bristol (GB); Krishna Coimbatore Balram, Bristol (GB); and Ruth Oulton, Bristol (GB)
Assigned to The University of Bristol, Bristol (GB)
Appl. No. 16/634,449
Filed by The University of Bristol, Bristol (GB)
PCT Filed Jul. 30, 2018, PCT No. PCT/GB2018/052154
§ 371(c)(1), (2) Date Jan. 27, 2020,
PCT Pub. No. WO2019/025771, PCT Pub. Date Feb. 7, 2019.
Claims priority of application No. 1712279 (GB), filed on Jul. 31, 2017.
Prior Publication US 2021/0088513 A1, Mar. 25, 2021
Int. Cl. G01N 33/543 (2006.01); A61L 2/025 (2006.01); C12M 1/34 (2006.01); C12Q 1/00 (2006.01); G01N 21/49 (2006.01); G01N 21/55 (2014.01); G01N 21/552 (2014.01); G01N 21/77 (2006.01); G01N 33/569 (2006.01)
CPC G01N 33/54373 (2013.01) [A61L 2/025 (2013.01); C12M 1/3446 (2013.01); C12Q 1/00 (2013.01); G01N 21/49 (2013.01); G01N 21/552 (2013.01); G01N 21/7703 (2013.01); G01N 33/56911 (2013.01); G01N 2021/556 (2013.01)] 16 Claims
OG exemplary drawing
 
1. An apparatus comprising:
a light source arranged to cast light toward a transparent coverslip supporting a bacteria binding volume to create an evanescent field, the bacteria binding volume being within the evanescent field;
a detector arranged to receive light from the bacteria binding volume and output data;
a processor programmed to determine a level of vibration of bacteria within the bacteria binding volume in three-dimensions from the output data;
the transparent coverslip arranged to support the bacteria binding volume;
the bacteria binding volume; and
a coverslip support arranged to support the coverslip, the coverslip support being arranged between the light source and the coverslip and having a hole to allow the light cast from the light source to pass to the transparent coverslip;
wherein the evanescent field is created on a surface of the transparent coverslip,
wherein the bacteria binding volume is supported directly on the transparent coverslip; and
wherein the transparent coverslip has no cladding.