US 12,405,270 B2
Method for magnetically labeling particles and labeling apparatus
Hiroshi Ito, Kyoto (JP); Masahiro Kozuka, Kyoto (JP); Takahide Inouchi, Kyoto (JP); and Shigeru Kitamura, Kyoto (JP)
Assigned to ARKRAY, Inc., Kyoto (JP)
Filed by ARKRAY, Inc., Kyoto (JP)
Filed on Jan. 6, 2022, as Appl. No. 17/569,551.
Application 17/569,551 is a division of application No. 15/720,554, filed on Sep. 29, 2017, granted, now 11,249,077.
Claims priority of application No. 2016-193792 (JP), filed on Sep. 30, 2016; application No. 2016-193797 (JP), filed on Sep. 30, 2016; and application No. 2017-188908 (JP), filed on Sep. 28, 2017.
Prior Publication US 2022/0128549 A1, Apr. 28, 2022
Int. Cl. G01N 33/543 (2006.01); B01D 29/05 (2006.01); G01N 33/574 (2006.01)
CPC G01N 33/54333 (2013.01) [B01D 29/05 (2013.01); G01N 33/54326 (2013.01); G01N 33/57488 (2013.01); G01N 2458/00 (2013.01)] 10 Claims
OG exemplary drawing
 
1. An apparatus for labeling particles with magnetic beads, comprising:
a filter portion a filter having a capture surface;
an introduction flow channel through which a suspension comprising magnetic beads is introduced into the filter portion;
an ejection flow channel that holds or rejects liquid of the suspension that has passed through the filter portion;
a liquid feeding means that supplies the suspension to the filter portion such that the particles are captured on the capture surface of the filter; and
a control unit that controls the liquid feeding means,
wherein the control unit is configured to control the liquid feeding means so as to
create a state in which both the capture surface and the other surface being opposite to the capture surface are in contact with the suspension comprising the magnetic beads,
from the state in which both the capture surface and the other surface of the filter are in contact with the suspension comprising the magnetic beads, reversely feed the suspension comprising the magnetic beads through the filter from the other surface of the filter toward the capture surface of the filter, and
pass the suspension comprising unreacted magnetic beads through the filter from the capture surface of the filter toward the other surface of the filter so that the unreacted magnetic beads are removed and the particles labeled with the magnetic beads are captured on the capture surface of the filter.