	US 12,405,265 B2
	Transient reporters and methods for base editing enrichment
David Brafman, Phoenix, AZ (US); Xiao Wang, Chandler, AZ (US); Nicholas Brookhouser, Tempe, AZ (US); Stefan Tekel, Tempe, AZ (US); and Kylie Standage-Beier, Phoenix, AZ (US)
Assigned to ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY, Scottsdale, AZ (US)
Filed by ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY, Scottsdale, AZ (US)
Filed on Jun. 14, 2021, as Appl. No. 17/347,360.
Claims priority of provisional application 63/038,220, filed on Jun. 12, 2020.
Prior Publication US 2021/0389303 A1, Dec. 16, 2021
Int. Cl. G01N 33/50 (2006.01); C07K 14/435 (2006.01); C12N 9/22 (2006.01); C12N 15/11 (2006.01); C12N 15/90 (2006.01)

CPC G01N 33/5073 (2013.01) [C07K 14/43595 (2013.01); C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12N 15/907 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01)]

1 Claim

1. A polynucleotide encoding one or more reporter polypeptides, the polynucleotide including a PAM site adjacent to a base that when edited causes a change in a function or characteristic of the one or more reporter polypeptides, wherein the polynucleotide comprises a sequence selected from the group consisting of: SEQ ID NO: 258; SEQ ID NO:259; and SEQ ID NO: 260; and wherein the one or more reporter polypeptides are selected from:

a reporter polypeptide comprising SEQ ID NO: 2, wherein the polypeptide comprises histidine at amino acid position number 67 and glycine at amino acid position number 73;

a reporter polypeptide comprising SEQ ID NO: 316; and

a reporter polypeptide comprising SEQ ID NO: 318.