US 12,405,264 B2
Electrophoretic system and method for analyte capture
Marlon Stoeckius, Stockholm (SE)
Assigned to 10x Genomics, Inc., Pleasanton, CA (US)
Filed by 10x Genomics, Inc., Pleasanton, CA (US)
Filed on Jan. 15, 2021, as Appl. No. 17/150,997.
Claims priority of provisional application 62/962,559, filed on Jan. 17, 2020.
Prior Publication US 2021/0223227 A1, Jul. 22, 2021
Int. Cl. G01N 33/487 (2006.01); B01L 3/00 (2006.01); G01N 27/447 (2006.01)
CPC G01N 33/48721 (2013.01) [B01L 3/5025 (2013.01); G01N 27/44791 (2013.01); B01L 2400/0421 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method for capturing analytes from one or more biological samples, the method comprising:
placing the one or more biological samples in contact with capture probes on a substrate, the one or more biological samples including the analytes;
inserting the substrate into a substrate holder of a substrate cassette;
subsequent to placing the one or more biological samples in contact with the capture probes on the substrate and inserting the substrate into the substrate holder, arranging the substrate cassette and the substrate to align one or more apertures of the substrate cassette with one or more substrate regions of the substrate and define one or more buffer chambers on the one or more substrate regions;
supplying buffers in the one or more buffer chambers;
arranging a cathode to place one or more electrode plates of the cathode within the one or more buffer chambers; and
generating electric fields between the one or more substrate regions and the one or more electrode plates to cause the analytes in the one or more biological samples to migrate toward the capture probes on the substrate, wherein the substrate functions as an anode that is removably coupled to the substrate cassette.