US 12,405,254 B2
Low temperature oligosaccharide analytical system and method
Tian Tian, Sunnyvale, CA (US); and Yan Liu, Palo Alto, CA (US)
Assigned to Dionex Corporation, Sunnyvale, CA (US)
Filed by DIONEX CORPORATION, Sunnyvale, CA (US)
Filed on Feb. 18, 2022, as Appl. No. 17/675,284.
Prior Publication US 2023/0266285 A1, Aug. 24, 2023
Int. Cl. G01N 30/72 (2006.01); G01N 30/16 (2006.01); G01N 30/30 (2006.01); G01N 30/32 (2006.01); G01N 30/64 (2006.01); G01N 30/02 (2006.01)
CPC G01N 30/7266 (2013.01) [G01N 30/16 (2013.01); G01N 30/30 (2013.01); G01N 30/32 (2013.01); G01N 30/64 (2013.01); G01N 2030/027 (2013.01); G01N 2030/326 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A method of analyzing an oligosaccharide sample, the method comprising:
injecting the oligosaccharide sample into a chromatography column of the chromatography system;
flowing a mobile phase into the chromatography column to separate the oligosaccharide sample into one or more analytes that elute off the chromatography column at different times;
flowing the mobile phase from the chromatography column into an electrochemical detector to detect one or more analytes;
flowing the mobile phase from the electrochemical detector into an ion removal device;
removing at least ions of one charge from the mobile phase in the ion removal device; and
flowing the mobile phase to a mass spectrometer;
wherein the chromatography column, electrochemical detector, and the ion removal device are all at a temperature of 15° C. or less;
wherein less than 5% of the oligosaccharide sample is epimerized.