US 12,405,220 B2
Chromatographic reader devices for biodetection
Antonia E. Schaefer, Lake Elmo, MN (US); Nicholas T. Gabriel, Grand Rapids, MN (US); Samuel J. Fahey, Woodbury, MN (US); Adam C. Nyland, St. Paul, MN (US); Thaine W. Fuller, Lakeland, MN (US); John M. Kruse, Minneapolis, MN (US); Nicholas G. Amell, Burnsville, MN (US); Glendon D. Kappel, Minneapolis, MN (US); and Patrick A. Mach, Shorewood, MN (US)
Assigned to 3M Innovative Properties Company, St. Paul, MN (US)
Appl. No. 18/024,202
Filed by 3M INNOVATIVE PROPERTIES COMPANY, St. Paul, MN (US)
PCT Filed Aug. 18, 2021, PCT No. PCT/IB2021/057615
§ 371(c)(1), (2) Date Mar. 1, 2023,
PCT Pub. No. WO2022/049439, PCT Pub. Date Mar. 10, 2022.
Claims priority of provisional application 63/074,875, filed on Sep. 4, 2020.
Prior Publication US 2023/0314330 A1, Oct. 5, 2023
Int. Cl. G01N 21/77 (2006.01); G06T 7/90 (2017.01)
CPC G01N 21/77 (2013.01) [G06T 7/90 (2017.01); G01N 2021/7759 (2013.01); G06T 2207/10024 (2013.01)] 6 Claims
OG exemplary drawing
 
1. A reader device comprising:
a camera board configured to capture image data associated with an output signal area of a biological chromatographic test strip;
processing circuitry in communication with the camera board, the processing circuitry being configured to:
identify, from the image data captured by the camera board, a control dot and a test dot displayed in the output signal area of the biological chromatographic strip, based on:
a search of the image data captured by the camera board for pixel groupings;
detecting, in a result of the search, two circles based on each respective circle of the two circles having a respective radius that falls within a predefined range between ten (10) pixels and fifty-five (55) pixels;
identifying a first circle of the two circles as the control dot; and
identifying a second circle of the two circles as the test dot;
determine, based on the image data captured by the camera board, (i) first colorimetric intensity information associated with the control dot identified in the output signal area of the biological chromatographic test strip, and (ii) second colorimetric intensity information associated with the test dot identified in the output signal area of the biological chromatographic test strip;
determine a ratio between the first colorimetric intensity information associated with the control dot and the second colorimetric intensity information associated with the test dot;
determine, based only on the ratio between the first colorimetric intensity information associated with the control dot and the second colorimetric intensity information associated with the test dot, a concentration of a target analyte in a test sample submitted via the biological chromatographic test strip; and
an interface in communication with the processing circuitry, the interface being configured to output data indicative of the concentration of the target analyte determined by the processing circuitry.