US 12,405,219 B2
Reusable SERS molecule detection apparatus and use method thereof
Lei Wang, Shandong (CN); Xia Wang, Shandong (CN); Qiuling Zhao, Shandong (CN); Maorong Wang, Shandong (CN); and Qingyue Zhang, Shandong (CN)
Assigned to QINGDAO UNIVERISTY OF SCIENCE AND TECHNOLOGY, Shandong (CN); and UNIVERSITY OF JINAN, Shandong (CN)
Appl. No. 18/832,667
Filed by QINGDAO UNIVERSITY OF SCIENCE AND TECHNOLOGY, Shandong (CN); and UNIVERSITY OF JINAN, Shandong (CN)
PCT Filed Sep. 5, 2023, PCT No. PCT/CN2023/116965
§ 371(c)(1), (2) Date Jul. 24, 2024,
PCT Pub. No. WO2024/066955, PCT Pub. Date Apr. 4, 2024.
Claims priority of application No. 202211184945.4 (CN), filed on Sep. 27, 2022.
Prior Publication US 2025/0102439 A1, Mar. 27, 2025
Int. Cl. G01N 21/65 (2006.01)
CPC G01N 21/658 (2013.01) 2 Claims
OG exemplary drawing
 
1. A molecule detection method based on SERS, using a reusable SERS molecule detection apparatus comprising:
two laminar glasses, at least one precious metal nano-film, at least one metal ring and molecules, wherein
the at least one precious metal nano-film is deposited on a surface of one of the laminar glasses to form a SERS substrate,
the at least one metal ring is deposited on a marginal surface of the SERS substrate, the at least one metal ring and the SERS substrate form a two-dimensional air cavity; and the at least one metal ring comprises an annular structure with a uniform thickness and is rectangular or circular; another of the laminar glasses is closed over the at least one metal ring after absorbing and fixing the molecules to be detected thereon, and
a thickness of the molecules to be detected does not exceed a thickness of the at least one metal ring, so as to reserve an air gap in between a layer of the molecules and the SERS substrate; wherein a thickness of the at least one metal ring is greater than thicknesses of typical molecules and the at least one metal ring comprises an annular structure with a uniform thickness and is rectangular or circular, and the method comprises the following steps:
Step 1: taking a laminar glass, depositing a layer of coarse precious metal nano-film on a surface of the laminar glass and forming an SERS substrate;
Step 2: depositing a metal ring of a predetermined thickness at a marginal area of the precious metal nano-film with a shield;
Step 3: attaching molecules to be detected on a surface of another laminar glass, closing the laminar glass over the metal ring so to have the molecules to be detected in a two-dimensional air cavity, and promise that the molecules to be detected will not contact a surface of the precious metal nano-film;
Step 4: injecting light excited by a Raman spectrometer, and a molecule SERS spectrum based on local electromagnetic reinforcement is obtained; and
Step 5: replacing the laminar glass carrying solution of the molecules to be detected, and repeating steps 3-4 until all the molecules to be detected have been detected.