US 12,405,194 B2
Facing and quality control in microtomy
Partha P. Mitra, New York, NY (US); Charles Cantor, New York, NY (US); Baris Yagci, New York, NY (US); David Kleinfeld, New York, NY (US); Cong Zhang, New York, NY (US); and Steven Smith, New York, NY (US)
Assigned to Clarapath, Inc., Hawthorne, NY (US)
Filed by Clarapath, Inc., New York, NY (US)
Filed on Feb. 22, 2021, as Appl. No. 17/182,133.
Claims priority of provisional application 63/134,399, filed on Jan. 6, 2021.
Claims priority of provisional application 62/980,194, filed on Feb. 22, 2020.
Claims priority of provisional application 62/980,203, filed on Feb. 22, 2020.
Claims priority of provisional application 62/980,202, filed on Feb. 22, 2020.
Claims priority of provisional application 62/980,201, filed on Feb. 22, 2020.
Prior Publication US 2021/0262907 A1, Aug. 26, 2021
Int. Cl. G01N 1/36 (2006.01); G01B 11/22 (2006.01); G01N 1/06 (2006.01); G01N 1/28 (2006.01); G01N 1/31 (2006.01); G01N 21/64 (2006.01); G01N 35/00 (2006.01); G06T 7/00 (2017.01); G06T 7/521 (2017.01)
CPC G01N 1/36 (2013.01) [G01B 11/22 (2013.01); G01N 1/06 (2013.01); G01N 1/286 (2013.01); G01N 1/312 (2013.01); G01N 21/6456 (2013.01); G01N 21/6486 (2013.01); G01N 35/00603 (2013.01); G01N 35/00613 (2013.01); G01N 35/00732 (2013.01); G06T 7/0014 (2013.01); G06T 7/521 (2017.01); G01N 2001/2873 (2013.01); G01N 2035/00039 (2013.01); G01N 2035/00861 (2013.01); G01N 2035/00881 (2013.01); G06T 2207/30024 (2013.01)] 12 Claims
OG exemplary drawing
 
1. A method for facing a tissue block comprising:
imaging a tissue block to generate imaging data of the tissue block, the tissue block comprising a tissue sample embedded in an embedding material;
estimating, based on the imaging data:
a depth profile of the tissue block, wherein the depth profile comprises a thickness of the embedding material to be removed to expose the tissue sample to a predetermined criteria; and
an expected outline, an expected size, and an expected shape of the tissue sample at a plurality of depths of the tissue block, the plurality of depths comprising a depth after the thickness of the embedding material is removed;
removing the thickness of the embedding material to expose the tissue sample to the predetermined criteria; and
confirming, based on the imaging data, when the thickness of the embedding material has been removed to expose the tissue sample to the predetermined criteria, by comparing an outline, size, or shape of the tissue sample in one or more sections cut from the tissue block to the expected outline, the expected size or the expected shape of the tissue sample at the depth estimated based on the imaging data.