US 12,404,605 B2
Method for diagnosing diseases using multiplex fluorescence and sequencing
Pavol Cekan, Bratislava (SK); and Evan Paul, Bratislava (SK)
Assigned to MultiplexDX, s.r.o., Bratislava (SK)
Appl. No. 17/282,725
Filed by MultiplexDX, s.r.o., Bratislava (SK)
PCT Filed Oct. 7, 2019, PCT No. PCT/EP2019/076989
§ 371(c)(1), (2) Date Apr. 2, 2021,
PCT Pub. No. WO2020/070325, PCT Pub. Date Apr. 9, 2020.
Claims priority of application No. 18198751 (EP), filed on Oct. 5, 2018; and application No. 100954 (LU), filed on Oct. 5, 2018.
Prior Publication US 2022/0002812 A1, Jan. 6, 2022
Prior Publication US 2023/0037279 A9, Feb. 2, 2023
Int. Cl. C40B 30/04 (2006.01); C12N 15/10 (2006.01)
CPC C40B 30/04 (2013.01) [C12N 15/1065 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A method for processing a biological sample, said method comprising:
(a) performing multiplex fluorescence in situ hybridization (FISH) on a biological sample to determine presence of mRNA species in the biological sample, wherein the biological sample is a tissue section sample which is formaldehyde-fixed, fresh frozen tissue section or formaldehyde-fixed, paraffin embedded tissue section;
(b) laser capturing cells of the biological sample having the mRNA species, wherein said laser capturing isolates the cells having the mRNA species from the biological sample, wherein said laser capturing comprises laser capture microdissection; and
(c) performing multiplex sequencing of the mRNA species from the isolated cells of the biological sample.