US 12,404,559 B2
Detecting Babesia species nucleic acid in a sample
Vanessa Bres, San Diego, CA (US); Deanna Self, Escondido, CA (US); and Jeffrey M. Linnen, Poway, CA (US)
Assigned to GEN-PROBE INCORPORATED, San Diego, CA (US)
Filed by Gen-Probe Incorporated, San Diego, CA (US)
Filed on Jan. 25, 2023, as Appl. No. 18/159,218.
Application 18/159,218 is a division of application No. 16/611,806, granted, now 11,667,978, previously published as PCT/US2018/036214, filed on Jun. 6, 2018.
Claims priority of provisional application 62/516,530, filed on Jun. 7, 2017.
Claims priority of provisional application 62/520,793, filed on Jun. 16, 2017.
Prior Publication US 2023/0227922 A1, Jul. 20, 2023
Int. Cl. C12Q 1/68 (2018.01); C07H 21/04 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6893 (2018.01)
CPC C12Q 1/6893 (2013.01) [C12Q 1/6806 (2013.01); C12Q 2600/16 (2013.01); C12Q 2600/166 (2013.01)] 13 Claims
 
1. A combination of at least two oligomers for determining the presence or absence of Babesia in a sample, said oligomer combination comprising first and second amplification oligomers for amplifying a target region of Babesia target nucleic acid, wherein
(a) the first amplification oligomer comprises a first target-hybridizing sequence that consists of SEQ ID NO:8, wherein the first amplification oligomer is a promoter primer or promoter provider further comprising a promoter sequence located 5′ to the first target-hybridizing sequence; and
(b) the second amplification oligomer comprises a second target-hybridizing sequence that consists of SEQ ID NO:34.