US 12,404,554 B2
Methods and kits relating to the capture of CA-IX positive exosomes
Antonio Chiesi, Siena (IT); Natasa Zarovni, Siena (IT); and Davide Zocco, Siena (IT)
Assigned to EXOSOMICS S.P.A., Siena (IT)
Appl. No. 16/623,360
Filed by EXOSOMICS S.P.A., Siena (IT)
PCT Filed Jun. 21, 2018, PCT No. PCT/EP2018/066604
§ 371(c)(1), (2) Date Dec. 16, 2019,
PCT Pub. No. WO2018/234463, PCT Pub. Date Dec. 27, 2018.
Claims priority of application No. 17177190 (EP), filed on Jun. 21, 2017.
Prior Publication US 2020/0291482 A1, Sep. 17, 2020
Int. Cl. C12Q 1/6886 (2018.01); G01N 33/543 (2006.01)
CPC C12Q 1/6886 (2013.01) [G01N 33/54306 (2013.01); C12Q 2600/156 (2013.01)] 6 Claims
OG exemplary drawing
 
1. A method for in vitro quantification of a tumour-related nucleic acid sequence present in exosomes from a biological fluid, the method comprising:
a) capturing exosomes from a sample of said biological fluid with an anti CA-IX antibody, wherein the sample comprises exosomes having a detectable level of a tumour-related nucleic acid sequence; and
b) detecting a level of the tumour-related nucleic acid sequence present in the exosomes captured in step a) for in vitro quantification of the tumour-related nucleic acid sequence from said biological fluid, wherein the tumour-related nucleic acid sequence comprises a gene sequence from a target gene selected from the group consisting of: a BRAF gene, a KRAS gene, a HERV gene, a LINE gene, a c-Myc gene and an AR gene.