US 12,404,552 B2
Methods of selecting antibodies and antibody fragments
Jiwu Wang, La Jolla, CA (US); Gerard G. Lambert, San Diego, CA (US); and Nathan C. Shaner, San Diego, CA (US)
Assigned to Allele Biotechnology & Pharmaceuticals, Inc., San Diego, CA (US)
Filed by Allele Biotechnology & Pharmaceuticals, Inc., San Diego, CA (US)
Filed on Jul. 16, 2016, as Appl. No. 15/212,215.
Claims priority of provisional application 62/194,102, filed on Jul. 17, 2015.
Prior Publication US 2017/0044608 A1, Feb. 16, 2017
Int. Cl. G01N 33/68 (2006.01); C12Q 1/6876 (2018.01)
CPC C12Q 1/6876 (2013.01) [G01N 33/6854 (2013.01); G01N 33/6857 (2013.01)] 19 Claims
 
1. A method for generating pharmaceutical batches comprising antibodies or antibody fragments specific for an immunogen, the method comprising expressing ex vivo a plurality of antibody variable regions encoded by DNA identified or selected from antibody variable region gene sequence information from genomic DNA of antibody-producing cells of a host animal immunized with the immunogen; which identification or selection does not comprise physical screening; wherein immunization of the host animal comprises presenting the immunogen in cells originated from the host animal or cells from the same species as the host animal; wherein said cells originated from the host animal or cells from the same species as the host animal are transfected to express the immunogen.