US 12,404,550 B2
Dark cycle sequencing
Eli N. Glezer, Del Mar, CA (US); Abrehet Abdu, San Diego, CA (US); and Timothy Looney, Austin, TX (US)
Assigned to Singular Genomics Systems, Inc., San Diego, CA (US)
Filed by Singular Genomics Systems, Inc., San Diego, CA (US)
Filed on Aug. 16, 2024, as Appl. No. 18/807,061.
Application 18/807,061 is a continuation of application No. 17/545,226, filed on Dec. 8, 2021, granted, now 12,123,054.
Application 17/545,226 is a continuation of application No. 17/127,308, filed on Dec. 18, 2020, granted, now 11,225,688, issued on Jan. 18, 2022.
Claims priority of provisional application 62/953,028, filed on Dec. 23, 2019.
Prior Publication US 2024/0401125 A1, Dec. 5, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6869 (2018.01)
CPC C12Q 1/6869 (2013.01) 22 Claims
OG exemplary drawing
 
1. A method of sequencing a nucleic acid molecule, said method comprising:
extending a primer hybridized to the nucleic acid molecule in a plurality of consecutive dark cycles; followed by extending the primer in a plurality of identifying cycles;
wherein each dark cycle comprises:
contacting the primer with a plurality of unlabeled nucleotides wherein an unlabeled nucleotide comprises a reversible terminator moiety, and incorporating with a polymerase the unlabeled nucleotide into the primer, and removing the reversible terminator moiety, wherein the unlabeled nucleotide comprising the reversible terminator moiety comprises the same nucleobase type in the plurality of consecutive dark cycles;
wherein each identifying cycle comprises:
contacting the primer with a plurality of sequencing nucleotides, and binding one sequencing nucleotide associated with a detectable label to the nucleic acid molecule, and detecting the label.