Methods and compositions for stabilizing nucleic acid-nucleotide-polymerase complexes
Morassa Mohseni Middleton, San Diego, CA (US); Mark C. Wallen, San Diego, CA (US); Pinar Iyidogan, San Diego, CA (US); Michael James Schmidt, San Diego, CA (US); Brittany A. Rohrman, La Jolla, CA (US); Ying Lin Liu, San Diego, CA (US); Fabian Block, San Diego, CA (US); and Arnold Oliphant, Morgan Hill, CA (US)
Assigned to Pacific Biosciences of California, Inc., Menlo Park, CA (US)
Filed by Pacific Biosciences of California, Inc., Menlo Park, CA (US)
Filed on Jan. 4, 2022, as Appl. No. 17/568,077.
Application 17/568,077 is a continuation of application No. 16/516,808, filed on Jul. 19, 2019, granted, now 11,242,557.
Application 16/516,808 is a continuation of application No. 16/355,361, filed on Mar. 15, 2019, granted, now 10,400,272, issued on Sep. 3, 2019.
Claims priority of provisional application 62/662,888, filed on Apr. 26, 2018.
Prior Publication US 2022/0290216 A1, Sep. 15, 2022
1. A composition, comprising a fluid, the fluid comprising a ternary complex and Li+, wherein the ternary complex comprises a primed template nucleic acid molecule comprising reversible terminator moiety, a polymerase, and a nucleotide cognate for the next correct base for the primed template nucleic acid molecule.