	US 12,404,547 B1
	Methods and systems for monitoring a recipient of an allograft
Robert Woodward, Pleasanton, CA (US); Marica Grskovic, Burlingame, CA (US); James Yee, San Mateo, CA (US); Mitch Nelles, Half Moon Bay, CA (US); and David Hiller, Brisbane, CA (US)
Assigned to CareDx, Inc.
Filed by CareDx, Inc., Brisbane, CA (US)
Filed on Nov. 4, 2024, as Appl. No. 18/936,839.
Application 18/936,839 is a continuation of application No. 18/455,456, filed on Aug. 24, 2023, abandoned.
Application 18/455,456 is a continuation in part of application No. 14/658,061, filed on Mar. 13, 2015, granted, now 11,767,559, issued on Sep. 26, 2023.
Claims priority of provisional application 61/953,582, filed on Mar. 14, 2014.
Int. Cl. C12P 19/34 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/686 (2018.01); G16B 20/00 (2019.01)

CPC C12Q 1/686 (2013.01) [C12Q 1/6806 (2013.01); G16B 20/00 (2019.02)]

22 Claims

1. A method for sequencing, comprising:

(a) providing a sample of a subject, wherein said sample comprises a plurality of cell-free nucleic acid molecules, and wherein said subject is a recipient of an allograft;

(b) isolating said plurality of cell-free nucleic acid molecules from said sample;

(c) amplifying said plurality of cell-free nucleic acid molecules to generate a plurality of amplified nucleic acid molecules;

(d) subjecting said plurality of amplified nucleic acid molecules, or derivatives thereof, to one or more amplification reactions using a plurality of primers to generate a plurality of deoxyribonucleic acid (“DNA”) molecules, wherein said plurality of primers target at least 100 independent polymorphisms; and

(e) sequencing said plurality of DNA molecules, or derivatives thereof,

wherein genotyping of said subject is not performed.