Methods of capturing multiple analytes on a spatial array
Augusto Manuel Tentori, Dublin, CA (US); Rajiv Bharadwaj, Pleasanton, CA (US); and Felice Alessio Bava, Rome (IT)
Assigned to 10x Genomics, Inc., Pleasanton, CA (US)
Filed by 10x Genomics, Inc., Pleasanton, CA (US)
Filed on Jan. 22, 2024, as Appl. No. 18/419,166.
Application 18/419,166 is a continuation of application No. 18/175,082, filed on Feb. 27, 2023, granted, now 11,933,957.
Application 18/175,082 is a continuation of application No. 17/665,169, filed on Feb. 4, 2022, granted, now 12,024,741.
Application 17/665,169 is a continuation of application No. 17/312,375, granted, now 12,180,543, previously published as PCT/US2019/065100, filed on Dec. 6, 2019.
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Prior Publication US 2024/0219701 A1, Jul. 4, 2024
mounting a first substrate comprising a tissue sample on a first member of a sample holder such that a first retaining mechanism on the first member retains the first substrate on the first member;
mounting a second substrate on a second member of the sample holder such that a second retaining mechanism on the second member retains the second substrate on the second member, the second substrate comprising an array comprising a plurality of capture probes, wherein a capture probe of the plurality of capture probes comprises: (i) a spatial barcode and (ii) a capture domain;
adding a reagent solution comprising a permeabilization agent to the first substrate and/or the second substrate;
operating a first actuator connected to the first member or the second member to reduce a distance between the second member and the first member in a direction orthogonal to a surface of the first substrate on which the tissue sample is positioned such that the biological sample is in contact with at least a portion of the array of capture probes via the reagent solution, wherein the first actuator is a linear actuator; and
capturing a first analyte, a second analyte, and/or a third analyte from the tissue sample on the array and permeabilizing at least a portion of the biological sample via the permeabilization agent of the reagent solution to facilitate transfer of a nucleic acid molecule from the biological sample for capture by the capture probe.
