US 12,404,538 B2
Compositions and methods for preserving DNA methylation
Sanford D. Markowitz, Pepper Pike, OH (US); Helen Moinova, Beachwood, OH (US); and Joseph Ready, Carrollton, TX (US)
Assigned to Case Western Reserve University, Cleveland, OH (US)
Appl. No. 17/927,525
Filed by Case Western Reserve University, Cleveland, OH (US)
PCT Filed May 26, 2021, PCT No. PCT/US2021/034274
§ 371(c)(1), (2) Date Nov. 23, 2022,
PCT Pub. No. WO2021/242872, PCT Pub. Date Dec. 2, 2021.
Claims priority of provisional application 63/172,279, filed on Apr. 8, 2021.
Claims priority of provisional application 63/030,407, filed on May 27, 2020.
Prior Publication US 2024/0417775 A1, Dec. 19, 2024
Int. Cl. C12N 15/10 (2006.01); A01N 1/00 (2006.01); C12Q 1/6806 (2018.01)
CPC C12Q 1/6806 (2013.01) [A01N 1/00 (2013.01); C12N 15/10 (2013.01); C12Q 2600/154 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A method of preserving the methylation pattern of methylated DNA molecule in a biological sample, comprising treating the biological sample with a storage solution, wherein the storage solution comprises methanol and tris, and wherein the methylation pattern is preserved at room temperature.
 
4. A method of preserving the methylation pattern of methylated DNA molecule in a biological sample, comprising treating the biological sample with a storage solution, wherein the storage solution comprises methanol and BHT, and wherein the methylation pattern is preserved at room temperature.