	US 12,404,531 B2
	Method for purifying (−)-Ambrox
Jean-Pierre Barras, Genèva (CH); Sylvie Morel, Prévessin-Moens (FR); and Eric Eichhorn, Zürich (CH)
Assigned to GIVAUDAN SA, Vernier (CH)
Appl. No. 18/018,360
Filed by Givaudan SA, Vernier (CH)
PCT Filed Jul. 29, 2021, PCT No. PCT/EP2021/071246 § 371(c)(1), (2) Date Jan. 27, 2023, PCT Pub. No. WO2022/023464, PCT Pub. Date Feb. 3, 2022.
Claims priority of application No. 2011823 (GB), filed on Jul. 30, 2020.
Prior Publication US 2023/0212624 A1, Jul. 6, 2023
Int. Cl. C12P 17/04 (2006.01); C12N 9/90 (2006.01)

CPC C12P 17/04 (2013.01) [C12N 9/90 (2013.01); C12Y 504/99017 (2013.01)]

10 Claims

1. A method for purifying a crude flavor or perfumery or cosmetic ingredient or intermediate, wherein the crude flavor or perfumery or cosmetic ingredient or intermediate is in solid form, the method comprising:

washing with an aqueous acid at a temperature less than the melting point of (−)-Ambrox;

washing with an aqueous surfactant at a temperature less than the melting point of (−)-Ambrox; and

washing with an aqueous alkali at a temperature less than the melting point of (−)-Ambrox:

wherein the crude flavor or perfumery or cosmetic ingredient or intermediate is made by a bioconversion process, wherein the crude flavor or perfumery or cosmetic ingredient or intermediate is separated from a bioconversion broth prior to the method of purification; and

wherein the method is a method for purifying crude (−)-Ambrox, crude (−)-Ambra-oxide, crude 3a-ethyl-6,6,9a-trimethyldodecahydronaphtho[2,1-b]furan, or crude (+)-Amberketal.