	US 12,404,530 B2
	Methods for producing a composition containing lipids from recombinant algae having high lipid productivity
Saheed Imam, La Jolla, CA (US); Eric R. Moellering, La Jolla, CA (US); Luke Peach, La Jolla, CA (US); Wiilliam F. Lambert, La Jolla, CA (US); and Kathleen Kwok, La Jolla, CA (US)
Assigned to Viridos, Inc., La Jolla, CA (US)
Filed by Viridos, Inc., La Jolla, CA (US)
Filed on Apr. 10, 2024, as Appl. No. 18/632,080.
Application 18/632,080 is a continuation of application No. 17/519,383, filed on Nov. 4, 2021, granted, now 11,976,315.
Claims priority of provisional application 63/110,301, filed on Nov. 5, 2020.
Prior Publication US 2024/0247290 A1, Jul. 25, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 7/64 (2022.01); C07K 14/405 (2006.01); C12N 1/12 (2006.01)

CPC C12P 7/64 (2013.01) [C07K 14/405 (2013.01); C12N 1/125 (2021.05)]

18 Claims

1. A method of producing a composition containing lipids comprising,

performing a deletion, disruption, or inactivation in an algal organism in a nucleic acid sequence having at least 90% sequence identity to SEQ ID NO: 15 and encoding a WD40 repeat containing protein having at least 90% sequence identity to the polypeptide of SEQ ID NO: 1 to produce a recombinant algal organism;

culturing the product recombinant algal organism in a culture medium, and thereby producing a composition containing lipids,

wherein the recombinant algal organism has higher lipid and/or higher biomass productivity versus a corresponding control algal host cell not having the deletion, disruption, or inactivation of the nucleic acid comprising the nucleotide sequence having at least 90% sequence identity to SEQ ID NO: 15 and encoding a WD40 repeat containing protein having at least 90% sequence identity to the polypeptide of SEQ ID NO: 1.