US 12,404,529 B2
Process for controlling organic acid ratios in a carbon dioxide bioconversion process
Ryan Senaratne, Fayetteville, AR (US); and Brandon Beard, Fayetteville, AR (US)
Filed by JUPENG BIO (HK) LIMITED, Hong Kong (CN)
Filed on Apr. 10, 2024, as Appl. No. 18/631,515.
Application 18/631,515 is a division of application No. 17/159,614, filed on Jan. 27, 2021, granted, now 11,981,950.
Claims priority of provisional application 62/967,220, filed on Jan. 29, 2020.
Prior Publication US 2024/0287552 A1, Aug. 29, 2024
Int. Cl. C12P 7/52 (2006.01); C12P 7/54 (2006.01)
CPC C12P 7/52 (2013.01) [C12P 7/54 (2013.01)] 19 Claims
 
1. A process comprising:
providing a gaseous substrate comprising CO2 to a bioreactor;
providing acetogenic bacteria and medium to the bioreactor to provide a fermentation broth;
providing sodium ions to the bioreactor through one or more sodium ion sources;
maintaining a pH of 6 or less in the fermentation broth; and
fermenting the gaseous substrate with the acetogenic bacteria in the fermentation broth to produce one or more organic acids;
wherein maintaining a pH of 6 or less in the fermentation broth provides a ratio of butyric acid to acetic acid concentration of 0.2 or more,
wherein the acetogenic bacteria includes a sodium translocating ATPase that is active during fermentation in the bioreactor, and
wherein the sodium ions are provided so that Na+ is maintained between 1000 to 11000 ppm (g/g) in the fermentation broth.