	US 12,404,528 B2
	Recombinant strain for producing shikimic acid, and construction method and use thereof
Liming Liu, Wuxi (CN); Qiang Ding, Wuxi (CN); Xiulai Chen, Wuxi (CN); Cong Gao, Wuxi (CN); Liang Guo, Wuxi (CN); Guipeng Hu, Wuxi (CN); and Jia Liu, Wuxi (CN)
Assigned to JIANGNAN UNIVERSITY, Wuxi (CN)
Filed by JIANGNAN UNIVERSITY, Wuxi (CN)
Filed on Mar. 12, 2022, as Appl. No. 17/693,349.
Application 17/693,349 is a continuation in part of application No. PCT/CN2021/135282, filed on Dec. 3, 2021.
Claims priority of application No. 202111425348.1 (CN), filed on Nov. 26, 2021.
Prior Publication US 2023/0332190 A1, Oct. 19, 2023
Int. Cl. C12P 7/42 (2006.01); C12N 1/21 (2006.01); C12N 9/10 (2006.01); C12N 9/12 (2006.01); C12N 9/88 (2006.01); C12N 15/70 (2006.01); C12R 1/19 (2006.01)

CPC C12P 7/42 (2013.01) [C12N 9/1022 (2013.01); C12N 9/1085 (2013.01); C12N 9/1205 (2013.01); C12N 9/88 (2013.01); C12N 15/70 (2013.01); C12R 2001/19 (2021.05); C12Y 202/01001 (2013.01); C12Y 205/01054 (2013.01); C12Y 207/01071 (2013.01); C12Y 402/03004 (2013.01)]

8 Claims

1. A recombinant strain for producing shikimic acid, wherein in the recombinant strain a shikimate kinase I coding gene aroK and a shikimate kinase II coding gene aroL are knocked out, and a target gene that regulates the asymmetric cell division and target genes that regulate the shikimic acid production are expressed,

the target gene that regulates the asymmetric cell division comprises a cytoskeletal protein PopZ coding gene popZ, and the target genes that regulate the shikimic acid production are 3-dehydroquinate synthase coding gene aroB, DAHP synthase coding gene aroG, and transketolase coding gene tktA,

wherein the recombinant strain is constructed with strain GL0002 as a host and the strain GL0002 was deposited in China Center for Type Culture Collection (Address: Bayi Road, Wuchang District, Wuhan City, Hubei Province) under the Accession No. CCTCC M 20251260 on Jun. 4, 2025.