US 12,404,519 B2
Antifungal plant proteins, peptides, and methods of use
Dilip Shah, St. Louis, MO (US)
Assigned to Donald Danforth Plant Science Center, St. Louis, MO (US)
Filed by Donald Danforth Plant Science Center, St. Louis, MO (US)
Filed on Jun. 16, 2023, as Appl. No. 18/336,772.
Application 16/277,157 is a division of application No. 14/888,011, granted, now 10,253,328, issued on Apr. 9, 2019, previously published as PCT/US2014/035786, filed on Apr. 29, 2014.
Application 18/336,772 is a continuation of application No. 17/304,156, filed on Jun. 15, 2021, abandoned.
Application 17/304,156 is a continuation of application No. 16/277,157, filed on Feb. 15, 2019, abandoned.
Claims priority of provisional application 61/817,415, filed on Apr. 30, 2013.
Prior Publication US 2023/0392161 A1, Dec. 7, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. A01H 5/00 (2018.01); A01H 1/00 (2006.01); A01N 65/20 (2009.01); C07H 21/04 (2006.01); C07K 14/415 (2006.01); C12N 15/00 (2006.01); C12N 15/82 (2006.01)
CPC C12N 15/8282 (2013.01) [A01H 1/1255 (2021.01); A01H 5/00 (2013.01); A01N 65/20 (2013.01); C07H 21/04 (2013.01); C07K 14/415 (2013.01); C12N 15/00 (2013.01)] 8 Claims
 
1. A polynucleotide comprising a nucleic acid fragment that encodes an antifungal protein comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 16 or SEQ ID NO: 17, wherein the amino acid residues corresponding to the defensin gamma core amino acid sequence at residues 33 to 44 of SEQ ID NO: 16 or SEQ ID NO: 17 comprise the amino acid sequences of SEQ ID NO:51 or SEQ ID NO:52 and are unmodified, wherein any substitutions of other said amino acid residues of SEQ ID NO: 16 or SEQ ID NO: 17 are conservative amino acid residue substitutions, and wherein the nucleic acid fragment is operably linked to a heterologous promoter and to a translational initiation codon that is in frame with the nucleic acid fragment that encodes the antifungal protein.