	US 12,404,518 B2
	*Expression of IPT7 from TSS* promoter increases root mass and carbon sequestration**
Jessica Vu, La Jolla, CA (US); Xuelin Wu, La Jolla, CA (US); and Joanne Chory, La Jolla, CA (US)
Assigned to Salk Institute for Biological Studies, La Jolla, CA (US)
Appl. No. 17/923,463
Filed by Salk Institute for Biological Studies, La Jolla, CA (US)
PCT Filed May 6, 2021, PCT No. PCT/US2021/031023 § 371(c)(1), (2) Date Nov. 4, 2022, PCT Pub. No. WO2021/226306, PCT Pub. Date Nov. 11, 2021.
Claims priority of provisional application 63/020,950, filed on May 6, 2020.
Prior Publication US 2023/0203520 A1, Jun. 29, 2023
Int. Cl. C12N 15/82 (2006.01)

CPC C12N 15/8262 (2013.01) [C12N 15/8243 (2013.01)]

19 Claims

1. A recombinant nucleic acid molecule, comprising:

a tetratricopeptide repeat (TPR)-domain suppressor of STIMPY (TSS) promoter operably linked to an isopentenyl-transferase 7 (IPT7) coding sequence, wherein the TSS promoter comprises at least 95% sequence identity to SEQ ID NO: 1, and the IPT7 coding sequence encodes a protein comprising at least 85% sequence identity to SEQ ID NO: 4; and

wherein the TSS promoter allows expression of the IPT7 coding sequence in mesophyll cells.